Acid phosphatase hydrolyses phosphate ester bonds under an optimum ... 5. Now you have to determine enzyme activities in all the original aliquots and for the fractions obtained from gel filtration.

This enzyme is a phosphatase ... by the nonspecific alkaline phosphatase of serum, the latter should be inactivated by the addition of ethylenediamine tetracetic acid before the determination.

Ribonucleic acid (RNA ... a ribose sugar, a phosphate group, and a nitrogenous base. Adjacent ribose nucleotide bases are chemically attached to one another in a chain via chemical bonds called ...

Acid phosphatase hydrolyses phosphate ester bonds under an optimum ... 5. Now you have to determine enzyme activities in all the original aliquots and for the fractions obtained from gel filtration.