In F2 screens, a mutagen, such as ethylnitrosourea (ENU), is used to generate hundreds of point mutations in the male pre-meiotic germ cells (spermatogonia). ENU-treated males are crossed to wild ...

They analyzed 878 pedigrees (murine family trees) comprising a total of 45,261 animals and detected 45,000 mutations induced ...

For instance, many recessive mutations segregate in each G1 animal in subsequent crosses, making it necessary to conduct complicated phenotype analyses. A second limitation to ENU mutagenesis is ...

Takahashi, professor of neurobiology and physiology at Northwestern and director of the newly established NIH Neurogenomics Center, random point mutations are introduced using the chemical ENU ...