With the new EpicIF technology, Bruker further advanced CellScape chemistry by expanding the range of compatible commercially available fluorophore-conjugated antibodies by nearly 10-fold ...
Two proteins in the sarcomeres, which give the tissue the characteristic striation pattern, were marked with antibodies and visualized by different fluorophore-conjugated antibodies: titin appears ...
Protein targets are often labelled with primary antibodies and fluorophore-conjugated secondary antibodies. However, the distance between the target and the dye-molecule reporter can be up to 30 nm.
We created a reagent spreadsheet that stated which vendors had which antibodies and in which colors. As for choosing which fluorophores to use, we needed to evaluate the autofluorescence of our ...
Clonal colonies of transfected CHO, HEK293 or myeloma cells secreting a recombinant protein of interest are probed in situ with target protein–specific, fluorophore-conjugated antibody ...
On occasion, it may be advantageous to use tetramers conjugated with APC, a more expensive fluorophore, which increases the cost of the tetramer to $900. The yield from a tetramer preparation is ...
Flow cytometry identifies cell types by reading fluorophore-bound antibodies attached to identifying cell markers, but the technique is limited by the 6 to 10 colors that can be detected without ...
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