In the last decade, two techniques have revolutionized fluorescence microscopy: the development of genetically encoded fluorophores, such as GFP 5,6 and the invention of two-photon microscopy 7.

To generate the GFP fusion protein ... or differential interference contrast (DIC) microscopy, using green light and/or adding a heat cut filter is sufficient to keep cells actively mitotic.

Due to the wave nature of light, any point source of light (even from a single GFP molecule with a size of 3 nm) is observed by the microscope as a small diffracted blur. As a result, the spatial ...