Gould (Division of Biochemistry and Molecular Biology, University of Glasgow) for the NH 2-terminal GLUT4 antibody. Disclosure: R.J.M.C. has received funding from Wellchild, the Royal College of ...
BSA and the protease inhibitors, phenylmethylsulfonyl fluoride, and all other reagent grade chemicals were purchased from Sigma (St. Louis, MO). Skeletal muscle cell growth medium (SkGM) (Cambrex ...
rabbit antibodies H-201 and S-19 specific for p110α and p110β (SantaCruz Biotechnology), rabbit anti-GLUT-1 and anti-GLUT4 (Chemicon International) and HRP-coupled secondary antibodies (Boehringer).