The Gibson Assembly® method has quickly become an invaluable tool for molecular cloning workflows due to its flexibility and efficiency. It allows for the cloning of multiple fragments directly into ...

Restriction enzymes are one of the most important tools in the recombinant DNA technology toolbox. But how were these enzymes discovered? And what makes them so useful? Aa Aa Aa When I come to the ...

This process is referred to as cloning. It uses restriction ... on the enzyme used. Then, enzymes called ligases "paste" the cut gene of interest into the cut plasmid. The result of step 2 is a ...