The hierarchical shotgun sequencing strategy involves several steps. First, a library is created by fragmenting the target genome and cloning it into vectors. Next, the genomic DNA fragments are ...
The shotgun phase of the Human Genome Project itself consisted of three steps: Obtaining a DNA clone to sequence Sequencing the DNA clone Assembling sequence data from multiple clones to determine ...
From February to September 2001, researchers from this public and private collaboration surged forward to collect the first genome's worth of Rat DNA data at an average pace of 15 million bases per ...