The Scientist2y
Brush Up: What Is Bisulfite Sequencing and How Do Researchers Use It to Study DNA Methylation?
1,2 Bisulfite conversion is the chemical reaction that occurs when scientists treat DNA with sodium bisulfite. In 1992, researchers found that the amination reaction of sodium bisulfite with ...
The Scientist2y
PBMCs: Mononucleated and Multipurposed
No matter the specific cell type, PBMCs are essential mediators of immune homeostasis and inflammation. 1 Bisulfite conversion is the chemical reaction that occurs when scientists treat DNA with ...
Nature5y
Capturing the human methylome
Pairing bisulfite conversion of the human genome with targeted enrichment and high-throughput sequencing allows a quantitative assessment of DNA methylation at base-pair resolution. A DNA molecule ...