This modified application of direct selection results in the production of clones in a sequence-ready format (in plasmid vectors ... genomic DNA using a QIAquick PCR purification kit.

Engineered enzymes play a crucial role in energy, materials, and medicine by enhancing natural functions or enabling new ...

E. coli colonies positive by PCR were grown overnight and plasmid extracted by midiprep plasmid purification (Promega). ExpiCHO-S cells (ThemoFisher) were revived from banked vials and passaged in ...

Here, we use a synthetic amphiphile to modulate the membrane mechanical properties of cell-derived vesicles and probe channel activation. Using precise membrane mechanical characterization approaches ...

Here we explore peptide inhibitors of Cdc20, a substrate-recognition subunit and activator of the E3 ubiquitin ligase the anaphase promoting complex/cyclosome (APC/C) that is essential in mitosis and ...

The best disk cloning software is one of the easiest ways to make a backup, or 'clone', of your solid state or hard disk drives, allowing you to restore all your files in the event of a corruption ...

T.J. Schall, M. Lewis, K.J. Koller, A. Lee, G.C. Rice, G.H.W. Wong, T. Gatanoga, et al., "Molecular cloning and expression of a receptor for human tumor necrosis ...

This paper describes the isolation, cloning, and molecular characterization of a receptor molecule for calcium ions located on the outer surface of certain types of cells. The reason it has received ...

Expert Rev Mol Diagn. 2012;12(7):731-754.

Bank card cloning occurs when criminals duplicate the data stored on the magnetic strip of your bank card such as a Visa Debit card issues by commercial Banks in Papua New Guinea. Criminals clone the ...

Natural resistance-associated macrophage proteins (NRAMPs) have been shown to transport a wide range of divalent metal ions, such as manganese (Mn), cadmium (Cd), and Iron (Fe). Iron is an essential ...