Genomic DNA was digested with 4 U of the Type IIB restriction enzyme (BcgI) for 3 h at 37°C. The digestion reaction was followed by ligation in a 10 μL mixture containing the digested DNA, T4 DNA ...
Like most other modification-dependent restriction endonucleases (MDREs ... For HhiV4I digests, reactions were carried out in NEB buffer 2.1 supplemented with 1 mM MnCl 2 (this enzyme is a Mn ...
A method for genetically barcoding Drosophila is developed and used to tag defined cell populations in vivo for single-cell transcriptomics experiments and to enable multiplexed behavioral analysis.
The team evaluated more than 4,000 clones before identifying an enzyme S N Ar1.3 that contains six mutations and is 160-fold more active than the parent enzyme.
The Bill will allow specified officers, including those from the police and Commercial Affairs Department, to issue restriction orders to banks if there is reasonable belief that account holders ...
Peer ReviewDownload a summary of the editorial decision process including editorial decision letters, reviewer comments and author responses to feedback. To address a wide range of genetic diseases, ...
This study presents important findings on the activity of two compounds, BKC and IBC, isolated from Psoralea corylifolia, which act synergistically to inhibit cancer cell proliferation. Using a ...
Department of Engineering Science, University of Oxford, Oxford OX1 3PJ, U.K.
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